Welcome
Pre- and Postprocessing
Domestication
Quick-Quality-Control
Mutagenesis
Point-Mutagenesis
Saturation-Mutagenesis
Welcome to the GoldenMutagenesis web tool
Read our publication
Golden Mutagenesis: An efficient multi-site-saturation mutagenesis approach by Golden Gate cloning with automated primer design
Pascal Püllmann, Chris Ulpinnis, Sylvestre Marillonnet, Ramona Gruetzner, Steffen Neumann & Martin J. Weissenborn
Publication as enhanced PDF on Springer Nature
How to cite?
Püllmann, P., Ulpinnis, C., Marillonnet, S. et al. Golden Mutagenesis:
An efficient multi-site-saturation mutagenesis approach by Golden Gate cloning with automated primer design.
Sci Rep 9, 10932 (2019). https://doi.org/10.1038/s41598-019-47376-1
AppVersion: 2020-08-28
Sequence Input
Configuration
Preview and Selection
Results
Domestication Configuration
Please select the restriction enzymes you want to domesticate.
BsaI
BbsI
Custom Recognition Site
Add
Remove
Selected Restriction Sites
Mutagenesis Configuration
Point-Mutagenesis with current mutations and sequence
Point-Mutagenesis with domesticated sequence
Saturation-Mutagenesis with domesticated sequence
Configuration
Results
Select .ab1/.abf
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Mutation Positions
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Sequence Input
Configuration
Preview and Selection
Results
Mutagenesis Configuration
Sequence Input
Configuration
Preview and Selection
Results
Mutagenesis Configuration
GoldenMutagenesis R Package
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GoldenMutagenesisWeb source code