Welcome to the GoldenMutagenesis web tool


Read our publication

Golden Mutagenesis: An efficient multi-site-saturation mutagenesis approach by Golden Gate cloning with automated primer design
Pascal Püllmann, Chris Ulpinnis, Sylvestre Marillonnet, Ramona Gruetzner, Steffen Neumann & Martin J. Weissenborn
Publication as enhanced PDF on Springer Nature

How to cite?

Püllmann, P., Ulpinnis, C., Marillonnet, S. et al. Golden Mutagenesis:
An efficient multi-site-saturation mutagenesis approach by Golden Gate cloning with automated primer design.
Sci Rep 9, 10932 (2019). https://doi.org/10.1038/s41598-019-47376-1

AppVersion: 2020-08-28



Domestication Configuration

Please select the restriction enzymes you want to domesticate.



Selected Restriction Sites

Mutagenesis Configuration


Mutation Positions

© 2020 Leibniz Institute of Plant Biochemistry

  • Die Leibniz-Gemeinschaft
  • Wissenschafts Campus Halle